Phospholipase D (PLD) Activity Colorimetric Assay Kit
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Catalog number
K725-100
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Price
Please ask
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Size
100 assays
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Description
Highly Sensitive Assay, HTS
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Summary
• Detection method- Absorbance (OD 570 nm) • Applications- - Measurement of PLD activity in various tissues/cells - Analyzing intracellular signaling - Screening of PLD inhibitors
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Detection Method
Absorbance (OD 570 nm)
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Applications
• Measurement of PLD activity in various tissues/cells • Analyzing intracellular signaling • Screening of PLD inhibitors
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Sample Type
• Animal tissues: liver, brain, kidney, etc. • Cell culture: Adherent or suspension cells
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Features Benefits
• Simple, rapid & convenient • The assay can detect phospholipase D (PLD) Activity less than 1 mU/ml in various sample types.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders. BioVision’s Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from Phosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Gene symbol
NAPEPLD
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Short name
Phospholipase D (PLD) Activity Colorimetric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Phospholipase D (PLD) Activity colorimetry test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Product images
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