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Alternative name1
Anti-p27kip1, phosphophorylated (Thr187)
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Alternative name2
p27kip1, phosphophorylated (Thr187)
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Alternative name3
p27kip1, phosphophorylated (Thr187)
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Alternative name4
p27kip1, phosphophorylated
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Alternative name5
Anti -p27kip1, phosphophorylated (Thr187)
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Other names
p27kip1; N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
N/A
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Clone
Polyclonal antibody
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Host organism
Host: Rabbit; Source: Human
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Species reactivity
Human
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Specificity and cross reactivity
Specific for the Thr-187 phosphorylated form of the cyclin-dependent kinase inhibitor p27KIP1. The antibody does not react with unphosphorylated p27 or with related CDK inhibitor proteins. This antibody can be used to detect endogenous phosphorylated-p27 by Western blotting if lysates are first immunoprecipitated with "plain" p27 antibody eg. Rb x p27, cat no. 71-9600 or Ms x p27, cat no.33-2800). Characterization of this antibody has been carried out primarily with purified recombinant p27 incubated in kinase reactions.(1)
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Purification method
Affinity Purified by affinity chromatography.
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Form Appearance
Supplied as a liquid in PBS, pH 7.4, containing 0.05% sodium azide.
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Concentration
N/A
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Storage and shipping
Store at 4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze productone should be stored at -20 degrees Celsius.. Aliquots are stable for at least 12 months productone should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested for
ELISA (EL/EIA), Western Blot (WB)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps