NKCC1 Antibody SLC12A2
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Catalog numberR31290
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenNKCC1 SLC12A2
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC-P, IHC-F
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Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting amounts. Titration of the NKCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis NKCC1 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP55011
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PurityAntigen affinity
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DescriptionSolute carrier family 12 (sodium/potassium/chloride transporters), member 2, also known as NKCC1 (Na-K-Cl Cotransporter 1), is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. NKCC1 is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
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ImmunogenAn amino acid sequence from the N-terminus of human NKCC1 (RIDHYRHTAAQLGEKLLRP) was used as the immunogen for this NKCC1 antibody (100% homologous in human, mouse and rat).
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StorageAfter reconstitution, the NKCC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSLC12A2, SLC12A2-DT
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Short nameAnti-NKCC1 SLC12A2
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to NKCC1 SLC12A2
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namesolute carrier family 12 member 2
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Synonyms gene name
- solute carrier family 12 (sodium/potassium/chloride transporter), member 2
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Solute carriers
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSLC12A2 divergent transcript
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Synonyms gene
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Synonyms gene name
- long intergenic non-protein coding RNA 1184
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Synonyms
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GenBank acession
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Locus
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Discovery year2014-01-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Divergent transcripts
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data