Neutrophil Elastase Antibody
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Catalog number
F41911-0.08ML
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Price
Please ask
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Size
0.08 ml
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Category
Antibody
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Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Neutrophil Elastase
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, ELISA
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Recommended dilutions
Western blot: 1:1000
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Notes
Titration of the Neutrophil elastase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This Neutrophil elastase antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P08246
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Purity
Antigen affinity
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Description
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
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Immunogen
A portion of amino acids 13-39 from the human protein was used as the immunogen for this Neutrophil elastase antibody.
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Storage
Aliquot the Neutrophil elastase antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
ELANE, SERPINB1
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Short name
Anti-Neutrophil Elastase
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to Neutrophil Elastase
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Alternative technique
antibodies
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Gene info
Gene info
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Identity
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Gene
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Long gene name
serpin family B member 1
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Synonyms gene
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Synonyms gene name
- serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1
- serpin peptidase inhibitor, clade B (ovalbumin), member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1993-07-27
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Entrez gene record
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Pubmed identfication
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Classification
- Serpin peptidase inhibitors
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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