Neutralite Avidin-FITC

• Catalog number
  MBS674999
• Price
  Please ask
• Size
  5x1 mg

• Products_type
  Antibody
• Products_short_name
  [Neutralite Avidin]
• Products_name_syn
  [Neutralite Avidin - FITC; Neutralite Avidin]
• Clonality
  Polyclonal
• Specificity
  d-Biotin
• Form
  Fluorescein (FITC) Conjugate
• Storage_stability
  The purified (UNLB) avidin is supplied as 5.0 mg protein in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), Cyanine 2 (CY 2), and Cyanine 5 (CY 5) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The Cyanine 3 (CY 3) conjugate is supplied as 0.1 mg in 0.2 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The beta -galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4, containing 0.1% sodium azide as preservative. Store at 2-8 degree C or long-term at -20 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
• Tested_application
  ELISA; Flow Cytometry; Immunoblot; Immunohistochemistry
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Neutralite   Avidin  
• Short name
  Neutralite Avidin-FITC
• Technique
  FITC, Fluorescein
• Host
  Chicken
• Label
  FITC
• Alternative name
  Neutralite Avidin-fluorecein
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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