MRCK beta, Active

Synonyms

MRCK, CDC42 binding protein kinase beta (DMPK-like)
Alternative_names

MRCK, CDC42 binding protein kinase beta (DMPK-like), DMPK-like beta, Myotonic dystrophy kinase-related CDC42-binding kinase beta
Description

Involved in the regulation of cytoskeleton reorganization, cell migration, wound healing and invasion
Recombinant

Yes
Source

Baculovirus (Sf9 insect cells)
Purity by SDS PAGE

≥90%
Assay

SDS-PAGE
Activity Specifications test method

The specific activity of MRCKB was determined to be 120 nmol /min/mg as per activity assay protocol.
Molecular Weight

73.0 kDa
Storage Temp

-80°C
Shipping

dry ice
Shelf Life

12 months
Concentration

0.1mg/ml
Appearance

Liquid
Physical form description

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Background Information

Myotonic Dystrophy Kinase-Related cdc42-binding kinase β (MRCKb) belongs to the DMPK subfamily (1). The myotonic dystrophy kinase-related kinases and myotonic dystrophy kinase-related Cdc42 binding kinase (MRCK) are effectors of RhoA and Cdc42, respectively, where they are involved in actin cytoskeletal reorganization and neurite outgrowth (2). Effects of the repeat expansion on the DMPK gene may be responsible for muscle and heart features of Myotonic Dystrophy.
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet