Mouse T1/ST2 Monoclonal Antibody rat anti-mouse T1/ST2 mAb-FITC Conjugated
-
Catalog number
MBS442003
-
Price
Please ask
-
Size
0.5 mL
-
-
Products_type
Antibody
-
Products_short_name
[T1/ST2 (IL-33R)]
-
Products_name_syn
[T1/ST2 (IL-33R) Monoclonal Antibody, FITC]
-
Products_gene_name
[T1/ST2]
-
Clonality
Monoclonal
-
Clone
[DJ8]
-
Specificity
This clone recognizes the membrane anchored murine T1M protein on the surface of T helper 2 cells and mast cells. T1M appears on fetal blood derived mast cell progenitors before they express the Fce RI, on IL-3-dependent bone marrow derived mast cells and on mature peritoneal mast cells. The antibody detects T1S protein consisting only of the extracellular portion of the protein, which is secreted from growth factor and proinflammatory cytokine-stimulated murine fibroblasts.
-
Form
The antibody is supplied in PBS, with 0.1% sodium azide as preservative and purified over protein G-sepharose. The characteristics of each lot are tested by FACS analysis with bone marrow derived mast cells.
-
Concentration
1mg/ml
-
Storage_stability
Store 2-8 degree C. Protect from the light
-
Tested_application
Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Rattus norvegicus
-
French translation
anticorps
-
Gene target
-
Gene symbol
CHST9, IL1RL1
-
Short name
Mouse T1/ST2 Monoclonal Antibody anti-mouse T1/ST2 mAb-FITC Conjugated
-
Technique
Antibody, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, Monoclonals or monoclonal antibodies, mouses
-
Host
mouse
-
Isotype
IgG1 (light chain not isotyped)
-
Label
FITC
-
Species
Mouse, Mouses, Rats
-
Alternative name
Mouse T1/ST2 monoclonal (antibody to-) rat Antibody tomouse T1/ST2 monoclonal-fluorecein coupled
-
Alternative technique
antibodies, murine, fluorescine
-
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
