Anti Mouse IgG1 Human Isotype Control-FITC
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Catalog numberICIGG1F-100
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Price:
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Size0,1 mg
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DescriptionAnti Mouse IgG1 Human Isotype Control-FITC is a mouse IgG1 monoclonal antibody type human isotype control, produced by [B11/6] clones, this reagent was conjugated with FITC format and reported for use in flow cytometric analysis as a negative control, it's used for testing the reliabilty of biological tests. Use the same concetration of the specific antibody of interest.
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CloneB11/6
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ConjugateFITC
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ApplicationFlow Cytometry Method
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FormulationPhosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
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ExpirationAfter 1 year
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Storage2-8°C, store in dark, do not freeze
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RemarkFor Recherche Use Only.
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Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody. Isotype or positive controls by peptides, antibodies and deactivated samples.
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PropertiesThis Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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ConjugationAnti-FITC Antibody
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Groupisotypes
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameAnti Mouse IgG1 Isotype Control-FITC
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Techniqueanti mouse, Control, Isotype, Mouse, anti, FITC, antibody to, anti mouse antibodies, controls, Fluorescein, Isotypes and controls, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse IgG1 Human Isotype Control Monoclonal Antibody [B11/6]-FITC
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Alternative techniquecontrol, controls, murine, antibodies, fluorescine
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Tissuecontrol
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data