Anti-RPA70 Antibody (8C3-D12-H10)

• Catalog number
  A1317-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  RPA70
• Target Alternative Name
  Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1, Single-stranded DNA-binding protein, RPA1
• Antibody Type
  Monoclonal
• Clone
  8C3-D12-H10
• Species Reactivities
  Human, Mouse, Rat, Monkey
• Immunogen Sequence
  Recombinant human RPA70 protein fragments expressed in E.coli
• Accession
  P27694
• Gene Id
  6117
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS (pH 7.4) containing with 0.02% sodium azide and 50%
• Purification
  Affinity purified
• Application
  WB, IP, IF
• Positive Control
  WB: Hela, 293T, C6, 3T3 and K562 cell lysates; WB: Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates; IP: HeLa cell lysates; IF: HeLa cells
• Application And Usages
  WB; 1:1000, IF; 1:100, IP
• Description
  Replication protein A 70 kDa DNA-binding subunit plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  RPA70   8C3-D12-H10  
• Gene symbol
  USE1, RP2, RPA1
• Short name
  Anti-RPA70 Antibody (8C3-D12-H10)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG2a
• Alternative name
  Antibody toRPA70 (antibody to-) (8C3-D12-H10)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:30882
• Gene
  USE1
• Long gene name
  unconventional SNARE in the ER 1
• Synonyms gene name
  • unconventional SNARE in the ER 1 homolog (S. cerevisiae)
• Synonyms
  • p31
  • SLT1
  • MDS032
  • D12
• Synonyms name
  • Q-SNARE
  • SNARE-like tail-anchored protein 1 homolog (S. cerevisiae)
• GenBank acession
  • AF220052
• Locus
  19p13.11
• Discovery year
  2007-08-20
• Entrez gene record
  55850
• Pubmed identfication
  • 16354670
  • 15029241
• RefSeq identity
  • NM_018467
• Classification
  • SNAREs
• VEGA ID
  OTTHUMG00000182723

Gene info

• Identity
  HGNC:10274
• Gene
  RP2
• Long gene name
  RP2 activator of ARL3 GTPase
• Synonyms gene name
  • retinitis pigmentosa 2 (X-linked recessive)
  • RP2, ARL3 GTPase activating protein
• Synonyms
  • TBCCD2
  • NME10
  • NM23-H10
• GenBank acession
  • AJ007590
• Locus
  Xp11.3
• Discovery year
  2001-06-22
• Entrez gene record
  6102
• Pubmed identfication
  • 18376416
  • 6325945
  • 9697692
  • 19852809
• RefSeq identity
  • NM_006915
• VEGA ID
  OTTHUMG00000021430
• Locus Specific Databases
  • Mutations of the RP2 Gene
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:10289
• Gene
  RPA1
• Long gene name
  replication protein A1
• Synonyms gene name
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
• Synonyms
  • REPA1
  • RPA70
  • HSSB
  • RF-A
  • RP-A
• GenBank acession
  • M63488
• Locus
  17p13.3
• Discovery year
  1993-12-14
• Entrez gene record
  6117
• Pubmed identfication
  • 8454588
• RefSeq identity
  • NM_002945
• Classification
  • Nucleotide excision repair
• VEGA ID
  OTTHUMG00000090579

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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