Mouse F4/80 Antigen, Macrophage, tissue fixed, Monocyte, Clone: CI:A3-1, Rat Mouse Monoclonal antibody-; IH/flow
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Catalog numberBMACIA3-1
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Price:
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Size0.5mg
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Alternative name 1Mouse F4/80 Antigen, Macrophage, tissue fixed, Monocyte, Clone: CI:A3-1, Rat Mab anti-; IH/flow
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Alternative name 2Mouse F4/80 Antigen, Macrophage, tissue fixed, Monocyte, Clone: CI:A3-1, Rat Mab antibody-; IH/flow
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Alternative name 3Mus musculus F4/80 Antigen, Macrophage, tissue fixed, Monocyte, Clone: CI:A3-1, Rattus norvegicus Mab antibody-; IH/flow
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Alternative name 4anti- F4/80 Antigen Macrophage tissue fixed Monocyte Clone: CI:A3-1
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Alternative name 5F4/80 Antigen Macrophage tissue fixed Monocyte Clone: CI:A3-1
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional descriptionAntigens are peptides or recombinant or native dependent on the production method. 6
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolSNAR-A3, CYP4A22-AS1, PCDHGA3, LPAR3, TCIRG1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1
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Short nameMouse F4/80 Antigen, Macrophage, tissue fixed, Monocyte, Clone: CI:A3-1, Mouse Monoclonal antibody-; IH/
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TechniqueMouse monoclonal, Antibody, antigen, tissue, Mouse, Flow, antibodies against human proteins, antibodies for, antigenes, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, tissues
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Hostmouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesMouse, Mouses, Rats
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Alternative nameMouse F4/80 protein, Macrophage, tissue fixed, Monocyte, clonality: CI:A3-1, Rat Mouse monoclonal (antibody to-)-; IH/flow
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Alternative techniqueantibodies, antigenes, tissues, murine
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Tissuetissue
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Gene info
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Identity
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Gene
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Long gene namesmall NF90 (ILF3) associated RNA A3
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Synonyms gene name
- small nuclear ILF3/NF90-associated RNA A3
- small ILF3/NF90-associated RNA A3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-06-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small NF90 (ILF3) associated RNAs
Gene info
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Identity
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Gene
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Long gene nameCYP4A22 antisense RNA 1
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Synonyms gene name
- CYP4A22 antisense RNA 1 (non-protein coding)
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Synonyms
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GenBank acession
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Locus
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Discovery year2012-02-02
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Pubmed identfication
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotocadherin gamma subfamily A, 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Clustered protocadherins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelysophosphatidic acid receptor 3
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Synonyms gene
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Synonyms gene name
- endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-01-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Lysophosphatidic acid receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameT cell immune regulator 1, ATPase H+ transporting V0 subunit a3
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Synonyms gene name
- T-cell, immune regulator 1
- T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a isoform 3
- T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
- T-cell immune regulator 1, ATPase H+ transporting V0 subunit a3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-type ATPase subunits
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data