Mouse IgG2a isotype control, monoclonal antibody (C1.18) (FITC conjugate)
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Catalog number
MBS565052
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Price
Please ask
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Size
0.2 mg
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Products_type
Antibody
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Products_short_name
[IgG2a]
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Products_name_syn
[IgG2a]
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Clonality
Monoclonal
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Clone
[C1.18]
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Reactivity
Species Independent
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Purity
Protein G-affinity purified.
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Form
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
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Storage_stability
Long term storage: 4 degree C Avoid freeze/thaw cycles. Protect from light.
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Tested_application
Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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Conjugation
Anti-FITC Antibody
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Group
isotypes
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Short name
Mouse IgG2a isotype control, monoclonal antibody (C1 18) (FITC conjugate)
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Technique
Antibody, Control, Isotype, Mouse, FITC, antibodies against human proteins, antibodies for, controls, Fluorescein, Isotypes and controls, Monoclonals or monoclonal antibodies, mouses
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Host
Mouse
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Isotype
IgG2a, IgG2a
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Mouse IgG2a isoform reference, monoclonal (antibody to-) (C1.18) (fluorecein conjugate)
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Alternative technique
antibodies, control, controls, murine, fluorescine
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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