Mouse IgG2a isotype control, monoclonal antibody (C1.18) (FITC conjugate)

  • Catalog number
    MBS565052
  • Price
    Please ask
  • Size
    0.2 mg
  • Products_type
    Antibody
  • Products_short_name
    [IgG2a]
  • Products_name_syn
    [IgG2a]
  • Clonality
    Monoclonal
  • Clone
    [C1.18]
  • Reactivity
    Species Independent
  • Purity
    Protein G-affinity purified.
  • Form
    Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
  • Storage_stability
    Long term storage: 4 degree C Avoid freeze/thaw cycles. Protect from light.
  • Tested_application
    Flow Cytometry (FC/FACS)
  • Properties
    If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • Conjugation
    Anti-FITC Antibody
  • Group
    isotypes
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Mouse IgG2a isotype control, monoclonal antibody (C1 18) (FITC conjugate)
  • Technique
    Antibody, Control, Isotype, Mouse, FITC, antibodies against human proteins, antibodies for, controls, Fluorescein, Isotypes and controls, Monoclonals or monoclonal antibodies, mouses
  • Host
    Mouse
  • Isotype
    IgG2a, IgG2a
  • Label
    FITC
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse IgG2a isoform reference, monoclonal (antibody to-) (C1.18) (fluorecein conjugate)
  • Alternative technique
    antibodies, control, controls, murine, fluorescine
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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