Mouse GDNF PicoKine ELISA Kit

Mouse GDNF PicoKine ELISA Kit is available 2 times from Boster labs

EK0935 | Mouse GDNF PicoKine ELISA Kitsize: 1x96 well plate | 579.76 USD

  • Catalog number
    EK0935
  • Supplier
  • Price
    579.76 USD
  • Size
    1x96 well plate
  • Gene symbol
    GDNF
1. Gene info
MeSH Data
  • Name
  • Concept
    Scope note:An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
Additional information
Product images

EK0935 | Mouse GDNF PicoKine ELISA Kitsize: 96wells/kit, with removable strips. | 549.17 USD

Ajax processing
  • ELISA's application
    For quantitative measurement of mouse GDNF in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
  • Gene tested
    murine GDNF
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.010 + 0.069 + 0.099 + 0.195 + 0.389 + 0.790 + 1.469 + 2.305
  • ELISA kit detection limits
    31.2pg/ml to 2000pg/ml
  • ELISA's sensitivity
    < 10pg/ml
  • Target of this ELISA
    Natural and recombinant mouse GDNF
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA's precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);138;716;1354;146;727;1379 ELISA standard deviations 6.07;36.52;86.66;10.51;53.8;113.1 CV(%);4.4;5.1;6.4;7.2;7.4;8.2
  • ELISA kit description
    GENTAUR's mouse GDNF ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for GDNF was used as capure antibodies on the ELISA test plate. The internal standards (sf21, S78-I211) and your samples are measured with a biotin conjugated polyclonal from goat specific for GDNF will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse GDNF in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse GDNF antibodies;1 ST0000-10;Lyophilized recombinant mouse GDNF standard;10ng/tube×2 AR1107;Biotinylated anti- mouse GDNF antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti-coagulation
    heparin or EDTA
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse GDNF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse GDNF standard solution and each sample be measured in duplicate.
  • Minutes of TMB incubation
    25-30
  • More gene info
    Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
  • Antigen
    sf21, S78-I211
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    Glial cell line-derived neurotrophic factor
  • Gene name
    glial cell derived neurotrophic factor
  • Alternative
    Astrocyte derived trophic factor;ATF;ATF1;ATF2;Glial cell line derived neurotrophic factor;HFB1 GDNF;hGDNF;HSCR3
  • Gen protein identity
    P48540
  • Entrez nr.
    14573
  • Gene Name
    Gdnf
  • Assay range
    31.2pg/ml-2000pg/ml
  • Immunogen
    Expression system for standard: sf21; Immunogen sequence: S78-I211
  • Storage & Transport Conditions
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
  • Cross-reactivity
    There is no detectable cross-reactivity with other relevant proteins.
  • Kit Components
    10ng/tube
  • Sample Type
    cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
  • Applications
    ELISA
  • Reactivity
    Mouse
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Mouse GDNF PicoKine ELISA Kit
  • Technique
    ELISA kit, ELISA, Mouse
  • Host
    mouse
  • Species
    Mouse
  • Alternative name
    Mouse glial cell derived neurotrophic factor PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas
  • Alternative to gene target
    glial cell derived neurotrophic factor, ATF1 and ATF2 and HFB1-GDNF and HSCR3, GDNF and IDBG-17169 and ENSG00000168621 and 2668, protein homodimerization activity, Extracellular, Gdnf and IDBG-128378 and ENSMUSG00000022144 and 14573, BT.58072 and IDBG-630047 and ENSBTAG00000005176 and 386587, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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