Mouse Anti-Rat VGLUT1 Monoclonal IgG1, Clone S28-9
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Catalog numberSMC-394D-PCP
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PricePlease ask
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Size100 µg
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CloneS28-9
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ImmunogenFusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
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Antibody s full descriptionMouse Anti-Rat VGLUT1 Monoclonal IgG1 Antibody, Clone: S28-9: PerCP
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameBNPI Antibody, SLC17A1 Antibody, SLC17A1/VGLUT1 Antibody, Solute Carrier family 17 member 7 Antibody, Vesicular glutamate transporter 1 Antibody, VGLUT 1 Antibody, Brain-specific Na(+)-dependent inorganic phosphate cotransporter Antibody
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Raised inMouse
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Antibody s targetVGLUT1
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers, Pumps/Transporters, Neurotransmitter Transporters, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~52kDa. No cross-reactivity against VGlut2.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Membrane , Cell Junction , Synapse
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextVGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
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Bibliography1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163. 2. Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.
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Released date16-May-2012
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PubMed number27000207
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Tested applicationsImmunohistochemistry
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Tested reactivityMouse
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NCBI numberNP_446311.1
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Gene number116638
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Protein numberQ62634
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (SMC-394). Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (SMC-394) at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas. Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (SMC-394). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (SMC-394) at 1:1000. Mouse Anti-VGLUT1 Antibody [S28-9] used in Immunohistochemistry (IHC) on Mouse spinal cord (SMC-394) Mouse Anti-VGLUT1 Antibody [S28-9] used in Western Blot (WB) on Rat brain membrane lysate (SMC-394)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolSLC17A7, RPS28, RNU6-9, PIRC45, PIRC47, PIRC38, PIRC43
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Short nameMouse Anti- VGLUT1 Monoclonal IgG1, Clone S28-9
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelPerCP
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat VGLUT1 monoclonal IgG1, clonality S28-9
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Alternative techniquemurine, antibodies
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Clone nameS28-9
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Gene info
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Identity
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Gene
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Long gene namesolute carrier family 17 member 7
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Synonyms gene name
- solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
- solute carrier family 17 (vesicular glutamate transporter), member 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-09-27
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Entrez gene record
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Pubmed identfication
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Classification
- Solute carriers
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameribosomal protein S28
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 9
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 45
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 47
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 38
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 43
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
