Mouse Anti-Rat TASK1 Potassium Channel Monoclonal IgG2b, Clone S374-48
#
-
Catalog numberSMC-473D-BI
-
Price:
-
Size100 µg
-
-
CloneS374-48
-
ImmunogenFusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical).
-
Antibody s full descriptionMouse Anti-Rat KCNK3 Monoclonal IgG2b Antibody, Clone: S374-48: Biotin
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other namePotassium channel subfamily K member 3 Antibody, KCNK3 Antibody, Acid sensitive potassium channel protein TASK 1 Antibody, Cardiac two pore background K(+) channel Antibody, cTBAK 1 Antibody, K2p3.1 Antibody, KCNK9 Antibody, OAT1 Antibody, potassium channel subfamily K member 3 Antibody, rTASK Antibody, TASK 1 Antibody, TBAK1 Antibody, TWIK related acid sensitive K+ channel Antibody, Two pore potassium channel KT3.1 Antibody, Two pore K(+) channel KT3.1 Antibody
-
Raised inMouse
-
Antibody s targetKCNK3
-
Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Tandem Pore Domain Potassium Channels
-
BrandnameNone
-
Antibodies applicationsWB, IHC, ICC/IF
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~50kDa. Does not cross-react with TASK3.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-473 was sufficient for detection of TASK1 Potassium Channel in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellMembrane
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextK+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54% identity with TASK-1, but less than 30% identity with TASK-2 and other tandem pore K+ channels.
-
Released date29-Oct-2013
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_203694.1
-
Gene number29553
-
Protein numberO54912
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (SMC-473). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (SMC-473) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) TASK1 Potassium Channel Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (SMC-473). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (SMC-473) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~50 kDa. Mouse Anti-TASK1 Potassium Channel Antibody [S374-48] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-473) Mouse Anti-TASK1 Potassium Channel Antibody [S374-48] used in Western Blot (WB) on Rat Brain Membrane (SMC-473)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus, Rattus norvegicus
-
Gene target
-
Gene symbolSNORD115-48, IGLV5-48, IGHV3-48, UTP18, RNU6-48P, KCNK3, HAUS8, SNORD115-45
-
Short nameMouse Anti- TASK1 Potassium Channel Monoclonal IgG2b, Clone S374-48
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG2b, IgG2b
-
LabelBiotin
-
SpeciesRat, Mouses, Rats
-
Alternative nameMouse Antibody toRat TASK1 Potassium Channel monoclonal IgG2b, clonality S374-48
-
Alternative techniquemurine, antibodies
-
Clone nameS374-48
-
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-48
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 5-48 (non-functional)
-
Synonyms gene name
- immunoglobulin lambda variable 5-48
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 3-48
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameUTP18 small subunit processome component
-
Synonyms gene
-
Synonyms gene name
- WD repeat domain 50
- UTP18 small subunit (SSU) processome component homolog (yeast)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2005-02-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- UTPb subcomplex
- WD repeat domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 48, pseudogene
-
Synonyms gene
-
Synonyms gene name
- RNA, U6 small nuclear 48
-
Locus
-
Discovery year2008-06-10
-
Entrez gene record
-
RefSeq identity
Gene info
-
Identity
-
Gene
-
Long gene namepotassium two pore domain channel subfamily K member 3
-
Synonyms gene name
- potassium channel, subfamily K, member 3
- potassium channel, two pore domain subfamily K, member 3
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1997-12-12
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Potassium two pore domain channel subfamily K
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameHAUS augmin like complex subunit 8
-
Synonyms gene
-
Synonyms gene name
- HEC1/NDC80 interacting, centrosome associated 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-12-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- HAUS augmin like complex
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-45
-
Synonyms
-
Locus
-
Discovery year2011-08-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data