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Clone
22B1
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Immunogen
Synthetic peptide
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Antibody s full description
Mouse Anti-Rat CaMKII Monoclonal IgG1 Antibody, Clone: 22B1: Alkaline Phosphatase
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody
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Raised in
Mouse
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Antibody s target
CaMKII
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Primary research fields
Cell Signaling, Post-translational Modifications, Phosphorylation
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, ELISA
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), IHC (1:100), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
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Antibody in cell
Cytoplasm , Mitochondrion , Nucleus , Cell Junction , Synapse , Presynaptic Cell Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
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Bibliography
1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.
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Released date
1-Nov-2007
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PubMed number
20148318
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Tested applications
Western Blot
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Tested reactivity
Mouse
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NCBI number
NP_037052.1
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Gene number
25400
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Protein number
P11275
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (SMC-125). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (SMC-125) at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech. Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (SMC-125). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (SMC-125) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (SMC-125). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (SMC-125) at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (SMC-125). Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (SMC-125) at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK. Mouse Anti-CaMKII Antibody [22B1] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rat dissociated hippocampal neurons (SMC-125) Mouse Anti-CaMKII Antibody [22B1] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-125) Mouse Anti-CaMKII Antibody [22B1] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-125) Mouse Anti-CaMKII Antibody [22B1] used in Western Blot (WB) on Mouse Ventricle lysates (SMC-125)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
