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Clone
S294A-6
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Immunogen
Fusion protein amino acids 219-655 of rat Brevican
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Antibody s full description
Mouse Anti-Rat Brevican Monoclonal IgG2B Antibody, Clone: S294A-6: ATTO 488
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
BCAN Antibody, BEHAB Antibody, CSPG7 Antibody, Brain enriched hyaluronan binding protein Antibody, Brevican core protein Antibody, Brevican core protein isoform 1 Antibody, Brevican core protein isoform 2 Antibody, Brevican proteoglycan Antibody, Chondroitin sulfate proteoglycan 7 Antibody, Chondroitin sulfate proteoglycan BEHAB Antibody, MGC13038 Antibody
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Raised in
Mouse
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Antibody s target
Brevican
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Primary research fields
Neuroscience, Neurodegeneration, Cell Signaling, Scaffolds
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Brandname
None
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Antibodies applications
WB, ICC/IF
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Antibody s reactivity
Mouse, Rat
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Antibody s dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~140kDa (and smaller due to proteolytic cleavage).
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-428 was sufficient for detection of Brevican in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Extracellular Space , Extracellular Matrix , Membrane , Lipid-Anchor , GPI-Anchor
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.
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Bibliography
1. Yamada H., Watanabe K., Shimonaka M. and Yamaguchi Y. (1994) J. Biol. Chem. 269: 10119-10126. 2. Yamada H., Watanabe K., Shimonaka M., Yamasaki M. and Yamaguchi Y. (1995) Biochem. Biophys. Res. Commun. 216: 957-963. 3. Rauch U., et al. (1997) Genomics 44: 15-21. 4. Zhang H., Kelly G., Zerillo C., Jaworski D.M. and Hockfield S. (1998) J. Neurosci. 7: 2370-2376. 5. Aspberg A., Adam S., Kostka G., Timpl R. and Heinegard D. (1999) J. Biol. Chem. 274: 20444-20449.
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Released date
15-Feb-2013
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_001028837.1
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Gene number
25393
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Protein number
P55068
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (SMC-428). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (SMC-428) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Brevican Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (SMC-428). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 µg). . Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (SMC-428) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa. Mouse Anti-Brevican Antibody [S294A-6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-428) Mouse Anti-Brevican Antibody [S294A-6] used in Western Blot (WB) on Rat Brain Membrane (SMC-428)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
