Mouse Anti-Mouse BK Beta3a Monoclonal IgG1, Clone S40B-18
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Catalog numberSMC-330D-P594
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Price:
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Size100 µg
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CloneS40B-18
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ImmunogenFusion protein amino acids 1-49 (unique N-terminus) of mouse BKBeta3a
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Antibody s full descriptionMouse Anti-Mouse KCNMB3 Monoclonal IgG1 Antibody, Clone: S40B-18: PE/ATTO 594
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameHBETA3 Antibody, KCNMB2 Antibody, KCNMBL Antibody, BKBeta3 Antibody, KCNMB3 Antibody, Calcium-activated potassium channel subunit beta-3 Antibody, BK channel subunit beta-3 Antibody, BKbeta3 Antibody, Hbeta3 Antibody, Calcium-activated potassium channel Antibody, subfamily M subunit beta-3 Charybdotoxin receptor subunit beta-3 Antibody, K(VCA)beta-3 Antibody, Maxi K channel subunit beta-3 Antibody, Slo-beta-3 Antibody
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Raised inMouse
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Antibody s targetKCNMB3
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Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Calcium-Activated Potassium Channels, Cancer, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~32 kDa. No cross-reactivity against BKBeta1, BKBeta2, BKBeta3b or BKBeta4.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-330 was sufficient for detection of BK Beta3a in 10 µg of COS cell (lysate) transiently transfected with BKbeta3a by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextBK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (1). A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia (2). This has major implications on other physiological processes in tissues other than the brain.
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Bibliography1. Wulf-Johansson H., et al. (2009) Brain Res. 1292: 1-13. 2. Lee U.S., and Cui J. (2009) J Physiol. 587(7): 1481-1489.
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_055222.3
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Gene number27094
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Protein numberQ9NPA1
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18 (SMC-330). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody (SMC-330) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Accumulation at the edges of the cell, ruffling edges. Western Blot analysis of Rat brain membrane lysate showing detection of KCNMB3 Potassium Channel protein using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18 (SMC-330). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody (SMC-330) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18 (SMC-330). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody (SMC-330) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Beautiful filaggrin-like staining. Mouse Anti-KCNMB3 Potassium Channel Antibody [S40B-18] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-330) Mouse Anti-KCNMB3 Potassium Channel Antibody [S40B-18] used in Western Blot (WB) on Rat brain membrane lysate (SMC-330) Mouse Anti-KCNMB3 Potassium Channel Antibody [S40B-18] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-330)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD114-18, PIRC96, SNORD116-18, IGLV2-18, IGHV1-18, ERVW-18, SNORD115-18, IGKV2D-18, IGKV2-18
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Short nameMouse Anti-Mouse BK Beta3a Monoclonal IgG1, Clone S40B-18
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelPE/ATTO 594
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse BK Beta3a monoclonal IgG1, clonality S40B-18
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Alternative techniquemurine, antibodies
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Clone nameS40B-18
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-18
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 96
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-18
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-18
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-18
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 18
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Synonyms gene name
- endogenous retrovirus group W, member 18
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-18
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-18 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2D-18
- immunoglobulin kappa variable 2D-18 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-18 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2-18
- immunoglobulin kappa variable 2-18 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data