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Clone
Trap1-6
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Immunogen
Purified recombinant TRAP1
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Antibody s full description
Mouse Anti-Human TRAP1 Monoclonal IgG2a Kappa Antibody, Clone: Trap1-6: PerCP
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Heat shock protein 75 Antibody, Heat shock protein 75 kDa Antibody, Heat shock protein 75 kDa mitochondrial Antibody, HSP 75 Antibody, HSP 90L Antibody, HSP75 Antibody, HSP90L Antibody, mitochondrial Antibody, TNF receptor associated protein 1 Antibody, TNFR associated protein 1 Antibody, TNFR-associated protein 1 Antibody, TRAP 1 Antibody, TRAP-1 Antibody, Trap1 Antibody, TRAP1_HUMAN Antibody, Tumor necrosis factor receptor associated protein Antibody, Tumor necrosis factor type 1 receptor associated protein Antibody, Tumor necrosis factor type 1 receptor-associated protein Antibody
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Raised in
Mouse
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Antibody s target
TRAP1
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Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking
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Brandname
None
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Antibodies applications
WB, ICC/IF
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Antibody s reactivity
Human
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Antibody s dilutions
WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~75kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-193 was sufficient for detection of Trap-1/HSP75 in 20 µg of Hela Cell lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Mitochondrion
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
The 90 kDa heat shock protein (HSP90) family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to be associated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting pre-sequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).
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Bibliography
1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312. 2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-46. 3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.
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Released date
5-May-2011
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_057376.2
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Gene number
10131
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Protein number
Q12931
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6 (SMC-193). Tissue: PC3M cells. Species: Human. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (SMC-193) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Mitotracker (red) mitochondria stain. DAPI (blue) nuclear stain. Western Blot analysis of Human Heat Shocked HeLa cell lysates showing detection of Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6 (SMC-193). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (SMC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Trap1 Antibody [Trap1-6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human PC3M cells (SMC-193) Mouse Anti-Trap1 Antibody [Trap1-6] used in Western Blot (WB) on Human Heat Shocked HeLa cell lysates (SMC-193)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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Gene target
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Short name
Mouse Anti- Trap1 Monoclonal IgG2a Kappa, Clone Trap1-6
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Technique
anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG2a Kappa, IgG2a
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Label
PerCP
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Species
Human, Humans, Mouses
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Alternative name
Mouse Antibody toHuman tumor necrosis factor receptor-associated protein 1 monoclonal IgG2a Kappa, clonality tumor necrosis factor receptor-associated protein 1-6
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Alternative technique
murine, antibodies
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Alternative to gene target
TNF receptor-associated protein 1, HSP 75 and HSP75 and HSP90L and TRAP-1, TRAP1 and IDBG-12290 and ENSG00000126602 and 10131,101929751, unfolded protein binding, nuclei, Trap1 and IDBG-129450 and ENSMUSG00000005981 and 68015, TRAP1 and IDBG-636097 and ENSBTAG00000020109 and 514472
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Clone name
Trap1-6
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