Mouse Anti-Human Trap1 Monoclonal IgG2a Kappa, Clone Trap1-6
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Catalog numberSMC-193D-A390
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Price:
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Size100 µg
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CloneTrap1-6
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ImmunogenPurified recombinant TRAP1
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Antibody s full descriptionMouse Anti-Human TRAP1 Monoclonal IgG2a Kappa Antibody, Clone: Trap1-6: ATTO 390
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameHeat shock protein 75 Antibody, Heat shock protein 75 kDa Antibody, Heat shock protein 75 kDa mitochondrial Antibody, HSP 75 Antibody, HSP 90L Antibody, HSP75 Antibody, HSP90L Antibody, mitochondrial Antibody, TNF receptor associated protein 1 Antibody, TNFR associated protein 1 Antibody, TNFR-associated protein 1 Antibody, TRAP 1 Antibody, TRAP-1 Antibody, Trap1 Antibody, TRAP1_HUMAN Antibody, Tumor necrosis factor receptor associated protein Antibody, Tumor necrosis factor type 1 receptor associated protein Antibody, Tumor necrosis factor type 1 receptor-associated protein Antibody
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Raised inMouse
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Antibody s targetTRAP1
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Primary research fieldsCancer, Heat Shock, Cell Signaling, Trafficking
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman
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Antibody s dilutionsWB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~75kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-193 was sufficient for detection of Trap-1/HSP75 in 20 µg of Hela Cell lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMitochondrion
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe 90 kDa heat shock protein (HSP90) family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to be associated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting pre-sequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).
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Bibliography1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312. 2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-46. 3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.
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Released date5-May-2011
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_057376.2
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Gene number10131
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Protein numberQ12931
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6 (SMC-193). Tissue: PC3M cells. Species: Human. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (SMC-193) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Mitotracker (red) mitochondria stain. DAPI (blue) nuclear stain. Western Blot analysis of Human Heat Shocked HeLa cell lysates showing detection of Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6 (SMC-193). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (SMC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Trap1 Antibody [Trap1-6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human PC3M cells (SMC-193) Mouse Anti-Trap1 Antibody [Trap1-6] used in Western Blot (WB) on Human Heat Shocked HeLa cell lysates (SMC-193)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Short nameMouse Anti- Trap1 Monoclonal IgG2a Kappa, Clone Trap1-6
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2a Kappa, IgG2a
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LabelATTO 390
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman tumor necrosis factor receptor-associated protein 1 monoclonal IgG2a Kappa, clonality tumor necrosis factor receptor-associated protein 1-6
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Alternative techniquemurine, antibodies
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Alternative to gene targetTNF receptor-associated protein 1, HSP 75 and HSP75 and HSP90L and TRAP-1, TRAP1 and IDBG-12290 and ENSG00000126602 and 10131,101929751, unfolded protein binding, nuclei, Trap1 and IDBG-129450 and ENSMUSG00000005981 and 68015, TRAP1 and IDBG-636097 and ENSBTAG00000020109 and 514472
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Clone nameTrap1-6
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data