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Clone
11C2
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Immunogen
A synthesized peptide derived from human Myosin Heavy Chain
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Antibody s full description
Mouse Anti-Human Myosin heavy chain Monoclonal IgG1 Antibody, Clone: 11C2: ATTO 390
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Cellular myosin heavy chain Antibody, Cellular myosin heavy chain type B Antibody, MYH 10 Antibody, Myosin 10 Antibody, Myosin heavy chain 10 Antibody, Myosin heavy chain non muscle 11b Antibody, Myosin-10 Antibody, Non muscle myosin heavy chain B Antibody
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Raised in
Mouse
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Antibody s target
Myosin heavy chain
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Brandname
None
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Antibodies applications
IHC, ICC/IF
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Antibody s reactivity
Human, Mouse, Rat, Fruit Fly (Drosophila melanogaster), Nematode (Caenorhabditis elegans)
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Antibody s dilutions
IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody buffer for storage
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
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Antibody s specificity
Detects ~220kDa, endogenous MHC proteins.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:100 dilution of SMC-258 was sufficient for detection of Myosin heavy chain in Nenmatodes using Goat Anti-Mouse IgG as the secondary antibody.
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Antibody in cell
Cell projection , Lamellipodium
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Myosins comprise a family of ATP-dependent motor proteins and are best known for their role in muscle contraction and their involvement in a wide range of other motility processes in eukaryotes. They are responsible for actin-based motility. The term was originally used to describe a group of similar ATPases found in the cells of both striated muscle tissue and smooth muscle tissue.
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Bibliography
1. Pollard TD et al. (1973) The Journal of Biological Chemistry. 248 (13): 4682–90.
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Released date
06-Sep-2016
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_005955.3
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Gene number
4628
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Protein number
P35580
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Myosin heavy chain Monoclonal Antibody, Clone 11C2 (SMC-258). Species: Nematode. Primary Antibody: Mouse Anti-Myosin heavy chain Monoclonal Antibody (SMC-258) at 1:100. Courtesy of: Provide by Tsinghua University. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Myosin heavy chain Monoclonal Antibody, Clone 11C2 (SMC-258). Species: Fruit Fly. Primary Antibody: Mouse Anti-Myosin heavy chain Monoclonal Antibody (SMC-258) at 1:100. Counterstain: F-actin (green). Courtesy of: Provide by NIBS. Mouse Anti-Myosin heavy chain Antibody [11C2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Nematode (SMC-258) Mouse Anti-Myosin heavy chain Antibody [11C2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Fruit Fly (SMC-258)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
