Mouse Anti-Guinea Pig IgG Antibody (H+L), Biotin Conjugated
-
Catalog number
bs-0358M-Biotin
-
Price
Please ask
-
Size
0.2ml
-
-
Category
Conjugated Secondary Antibody
-
Conjugation
Biotin
-
Host Organism
Mouse
-
Target antigen
Guinea Pig IgG
-
Modification
Unmodified
-
Modification site
None
-
Clonality
Polyclonal antibody
-
Clone
Polyclonal antibody
-
Concentration
1ug/ul
-
Tested applications
WB, ELISA, IHC-P, IHC-F
-
Recommended dilutions
WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)
-
Cross reactivity
Guinea Pig
-
Background of the target antigen
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
-
Purification method
This antibody has been purified via Protein A.
-
Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
-
Properties
If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Additional conjugation
Biotinylated
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Pigs and the smaller guinea pigs are frequent used as models for humans.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Short name
Anti-Guinea Pig IgG
-
Technique
Antibody, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, IgGs, mouses
-
Host
mouse
-
Isotype
IgG, IgG
-
Label
Biotin
-
Species
Guinea Pig, Guinea Pigs, Mouses, Pigs and Guinea pigs
-
Alternative name
Mouse Anti-Guinea Pig IgG
-
Alternative technique
antibodies, murine, immunoglobulins
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products