Mouse Anti-4-Hydroxynonenal (4-HNE) Monoclonal IgG1, Clone 12F7

• Catalog number
  SMC-511D-A488
• Price
  Please ask
• Size
  100 µg

• Clone
  12F7
• Immunogen
  Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
• Antibody s full description
  Mouse Anti- 4-Hydroxynonenal Monoclonal IgG1 Antibody, Clone: 12F7: ATTO 488
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  4-Hydroxynonenal Antibody, 4-Hydroxy Nonenal (4-HNE) Antibody, 4-Hydroxy Nonenal Antibody, 4-HNE Antibody, 4-hydroxy Hexenal Antibody, HNE Antibody, 4-hydroxy-2-nonenal Antibody
• Raised in
  Mouse
• Antibody s target
  4-Hydroxynonenal
• Primary research fields
  Cancer, Oxidative Stress, Lipid peroxidation
• Brandname
  MOLECULAR SIGNATURE®
• Antibodies applications
  WB, ICC/IF, ELISA
• Antibody s reactivity
  Species Independent
• Antibody s dilutions
  WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  A 1:1000 dilution of SMC-511 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Antibody in cell
  See included datasheet or contact our support service
• Tissue specificity
  See included datasheet or contact our support service
• Released date
  23-Mar-2017
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  Refer to NCBI
• Gene number
  Refer to GenBank
• Protein number
  Refer to Swiss-Prot
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7 (SMC-511). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (SMC-511) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin Alex Fluor 633 F-Actin stain (E,F) 4-Hydroxynonenal Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal-BSA using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (SMC-511). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (SMC-511) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Mouse Anti-4-Hydroxynonenal Antibody [12F7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-511) Mouse Anti-4-Hydroxynonenal Antibody [12F7] used in Western Blot (WB) on 4-hydroxy-nonenal-BSA Conjugate (SMC-511)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  4-Hydroxynonenal   4-HNE   Monoclonal   IgG1   12F7  
• Gene symbol
  MIR1302-4, PIRC18, ELANE, PIRC16, SNORD114-4, IGKV2OR2-4, MIR941-4, PIRC19
• Short name
  Mouse Anti-4-Hydroxynonenal (4-HNE) Monoclonal IgG1, Clone 12F7
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  ATTO 488
• Species
  Mouse, Mouses
• Alternative name
  Mouse Antibody to4-Hydroxynonenal (4-HNE) monoclonal IgG1, clonality 12F7
• Alternative technique
  murine, antibodies
• Clone name
  12F7

Gene info

• Identity
  HGNC:35296
• Gene
  MIR1302-4
• Long gene name
  microRNA 1302-4
• Synonyms gene
  • MIRN1302-4
• Synonyms
  • hsa-mir-1302-4
• Locus
  2q33.3
• Discovery year
  2008-11-03
• Entrez gene record
  100302130
• RefSeq identity
  • NR_031633
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:37522
• Gene
  PIRC18
• Long gene name
  piwi-interacting RNA cluster 18
• Locus
  4
• Discovery year
  2009-11-05
• Entrez gene record
  100313906
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:3309
• Gene
  ELANE
• Long gene name
  elastase, neutrophil expressed
• Synonyms gene
  • ELA2
• Synonyms gene name
  • elastase 2, neutrophil
• Synonyms
  • NE
  • HNE
  • HLE
  • PMN-E
• Synonyms name
  • neutrophil elastase
  • leukocyte elastase
  • medullasin
  • PMN Elastase
  • polymorphonuclear leukocyte elastase
• Locus
  19p13.3
• Discovery year
  1989-09-08
• Entrez gene record
  1991
• Pubmed identfication
  • 2902087
• RefSeq identity
  • NM_001972
• Classification
  • Granule associated serine proteases of immune defence
  • Complement system regulators and receptors
• VEGA ID
  OTTHUMG00000181839
• Locus Specific Databases
  • ELA2base: Mutation registry for Cyclic and congenital neutropenia
  • LRG_57

Gene info

• Identity
  HGNC:37520
• Gene
  PIRC16
• Long gene name
  piwi-interacting RNA cluster 16
• Locus
  4
• Discovery year
  2009-11-05
• Entrez gene record
  100313849
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:32992
• Gene
  SNORD114-4
• Long gene name
  small nucleolar RNA, C/D box 114-4
• Synonyms
  • 14q(II-4)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767580
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003196.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5809
• Gene
  IGKV2OR2-4
• Long gene name
  immunoglobulin kappa variable 2/OR2-4 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2/OR2-4
  • immunoglobulin kappa variable 2/OR2-4 pseudogene
• Synonyms
  • IGKV2/OR2-4
• GenBank acession
  • X51883
• Locus
  2q11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28858
• Pubmed identfication
  • 2111145
• Classification
  • Immunoglobulin kappa (IGK) orphons

Gene info

• Identity
  HGNC:33687
• Gene
  MIR941-4
• Long gene name
  microRNA 941-4
• Synonyms gene
  • MIRN941-4
• Synonyms
  • hsa-mir-941-4
• Locus
  20q13.33
• Discovery year
  2007-10-24
• Entrez gene record
  100126330
• RefSeq identity
  • NR_040032
• Classification
  • MicroRNAs

Gene info

• Identity
  HGNC:37523
• Gene
  PIRC19
• Long gene name
  piwi-interacting RNA cluster 19
• Locus
  4
• Discovery year
  2009-11-05
• Entrez gene record
  100313926
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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