MAPKAPK5(Thr182) Antibody, ALEXA FLUOR 647

• Catalog number
  bs-5504R-A647
• Price
  Please ask
• Size
  100ul

• Long name
  MAPKAPK5(Thr182) Polyclonal Antibody, ALEXA FLUOR 647 Conjugated
• Also known as
  Anti-MAPKAPK5 Thr182 PAb ALEXA FLUOR 647
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 647
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  MAPKAPK5 Thr182
• Specificity
  This is a highly specific antibody against MAPKAPK5 Thr182.
• Modification
  Phosphorylation
• Modification Site
  Thr182
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  160-210/473
• Subcellular location
  Cytoplasm, Nucleus
• Source
  KLH conjugated synthetic phosphopeptide derived from human MAPKAPK5 around the phosphorylation site of Thr182
• Gene ID Number
  8550
• Swiss Prot
  Q8IW41
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  650nm/665nm
• Synonyms
  MK5; MK-5; PRAK; MAPKAP-K5; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase; MAPKAPK5
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-MAPKAPK5 Thr182 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 647
• French translation
  anticorps

• Gene target
  MAPKAPK5   Thr182  
• Gene symbol
  MAPKAPK5-AS1, MAPKAPK5
• Short name
  Anti-MAPKAPK5 Thr182
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 647
• Alternative name
  MAPKAPK5(Thr182) Polyclonal Antibody, ALEXA FLUOR 647
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:24091
• Gene
  MAPKAPK5-AS1
• Long gene name
  MAPKAPK5 antisense RNA 1
• Synonyms gene
  • C12orf47
• Synonyms gene name
  • chromosome 12 open reading frame 47
  • MAPKAPK5 antisense RNA 1 (non-protein coding)
• Synonyms
  • FLJ39616
• Locus
  12q24.12
• Discovery year
  2006-01-23
• Entrez gene record
  51275
• Pubmed identfication
  • 12477932
• RefSeq identity
  • NR_015404
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000141273

Gene info

• Identity
  HGNC:6889
• Gene
  MAPKAPK5
• Long gene name
  MAPK activated protein kinase 5
• Synonyms gene name
  • mitogen-activated protein kinase-activated protein kinase 5
• Synonyms
  • PRAK
  • MK5
• GenBank acession
  • AF032437
• Locus
  12q24.12-q24.13
• Discovery year
  1999-03-26
• Entrez gene record
  8550
• Pubmed identfication
  • 9628874
• RefSeq identity
  • NM_139078
• Classification
  • MAPK activated protein kinases
• VEGA ID
  OTTHUMG00000169605

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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