M’Fadyean Stain (Polychrome Methylene Blue)
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Catalog number
S047-20X500ML
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Price
Please ask
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Size
20X500ML
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Description
M’Fadyean Stain (Polychrome Methylene Blue) is a readymade stain reported to be used for capsule staining of Bacillus anthracis
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Application
For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.
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Classification
Capsule Stains
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Composition
Methylene blue 0.300 gm 95% ethyl alcohol 30.000 ml Potassium hydroxide 0.010 gm Distilled water 100.000 ml
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Appearance
Deep violet coloured solution
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Clarity
Clear without any particles.
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Microscopic Examination
Capsule staining is carried out using M'Fadyean stain and staining characteristic is observed under microscope using oil immersion lens.
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Results
Bacillus anthracis ATCC 14578 : Pink capsule seen surrounding the deep bluish bacilli. Bacillus cereus ATCC 10876 : Deep bluish bacilli seen.
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Storage
Store below 30°C in tightly closed container and away from bright light.
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Shelf Life
Use before expiry date on label.
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Properties
Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
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Conjugation
Blue Substrate
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