LIF, murine recombinant
#
-
Catalog number4924-1000
-
Price:
-
Size1 mg
-
-
SynonymsLeukemia Inhibitory Factor
-
Alternative_namesLeukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, INN=Emfilermin
-
DescriptionA cytokine that affects cell growth by inhibiting differentiation
-
RecombinantYes
-
SourceE. coli
-
Purity by SDS PAGE≥95%
-
AssaySDS-PAGE
-
Purity≥95%
-
Molecular Weight20.0 kDa
-
Storage Temp-20°C
-
ShippingGel pack
-
Shelf Life12 months
-
AppearanceLyophilized protein
-
Physical form descriptionLyophilized from sterile solution containing 20 mM phosphate buffer, pH 7.4 and 0.02% Tween-20
-
Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile H₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffers.
-
Background InformationLeukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Leukemia Inhibitory Factor (LIF) Murine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.
-
Amino acid sequenceMSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQR KKLGCQLLGTYKQVISVVVQAF
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
Additional sourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolLIF-AS1, LIF-AS2, LIF
-
Short nameLIF, murine recombinant
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Alternative nameleukemia inhibitory factor, murine Rec.
-
Alternative techniquerec
-
Alternative to gene targetleukemia inhibitory factor, CDF and DIA and HILDA and MLPLI, LIF and IDBG-3968 and ENSG00000128342 and 3976, growth factor activity, Extracellular, Lif and IDBG-142317 and ENSMUSG00000034394 and 16878, LIF and IDBG-636335 and ENSBTAG00000007424 and 280840
-
Gene info
-
Identity
-
Gene
-
Long gene nameLIF antisense RNA 1
-
Synonyms
-
Locus
-
Discovery year2017-04-28
-
Pubmed identfication
-
Classification
- Antisense RNAs
Gene info
-
Identity
-
Gene
-
Long gene nameLIF antisense RNA 2
-
Locus
-
Discovery year2020-10-07
-
Entrez gene record
-
RefSeq identity
-
Classification
- Antisense RNAs
Gene info
-
Identity
-
Gene
-
Long gene nameLIF interleukin 6 family cytokine
-
Synonyms gene name
- leukemia inhibitory factor
- LIF, interleukin 6 family cytokine
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1989-06-11
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Receptor ligands
- Interleukin 6 type cytokine family
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data