Lhcb2, LHCII type II chlorophyll a/b-binding protein

• Catalog number
  AS01 003
• Price
  Please ask
• Size
  200 µl

• Available ordering format
  Lyophilized in PBS pH 7.4
• Immunogen
  BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb2 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb2.1 UniProt: Q9SHR7, TAIR: AT2G05100, Lhcb2.2 UniProt: Q9S7J7, TAIR:AT2G05070, Lhcb2.3 UniProt:Q9XF87, TAIR:AT3G27690
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Total IgG
• How to reconstitute
  For reconstitution add 200 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), immunoprecipitation (IP)
• Connected products
  AS01 002 | Lhcb3 | LHCII type III chlorophyll a/b-binding proteinAS01 004 | Lhcb1 | LHCII type I chlorophyll a/b-binding proteinAS13 2705 | Lhcb2-P | LHCII type II chlorophyll a/b-binding protein, phosphorylatedPlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1 : 5000 with standard ECL (WB), 5 µl of antibody solution (IP)
• Molecular weight expected аpparent
  28.6 | 25 kDa for Arabidopsis thaliana
• Verified reactivity
  Arabidopsis thaliana, Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Miscanthus x giganteus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Zea mays, Physcomitrella patens, Chlamydomonas reinhardtii
• Possible reactivity
  angiosperms (monocots and dicots), gymnosperms, mosses, green algae
• No reactivity
  no confirmed exceptions from predicted reactivity known at the moment
• Supplementary information
  Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.Protein is processed into mature form (Jansson 1999).
• References
  Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015. Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113. Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29. Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675. Spence et al. (2014). Transcriptional responses indicate maintenance of photosynthetic proteins as key to the exceptional chilling tolerance of C4 photosynthesis in Miscanthus × giganteus. J Exp Bot. 2014 Jul;65(13):3737-47. doi: 10.1093/jxb/eru209. Epub 2014 Jun 22. Saito et al. (2014). Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes. FEBS Lett. 2014 May 8. pii: S0014-5793(14)00317-2. doi: 10.1016/j.febslet.2014.04.031. Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.
• Scientific context
  The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476.
• Notes
  Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.
• Protein number
  Q9SHR7 , Q9S7J7 , Q9XF87 , Q9XF86
• TAIR number
  AT2G05100
• Hyperlink

• Gene target
  Lhcb2   LHCII   chlorophyll   a/b-binding   protein  
• Short name
  Lhcb2, LHCII II chlorophyll a/b-binding protein
• Alternative name
  Lhcb2, LHCII classification II chlorophyll a/b-binding protein

Similar products