Lens culinaris (LcH, Lentil) (FITC)
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Catalog number
MBS618451
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Price
Please ask
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Size
5x2 mL
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Products_type
Antibody
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Products_short_name
[Lens culinaris]
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Products_name_syn
[Anti -Lens culinaris (LcH, Lentil) (FITC)]
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Other_names
[lipid transfer protein 3]
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Clonality
Polyclonal
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Specificity
Recognizes Lens culinaris
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Purity
Purified Delipidated antisera purified by ammonium sulfate precipitation.
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Form
Supplied as 1ml of antibody in PBS, pH 7.2, 0.1% sodium azide, 25% glycerol.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Short name
Lens culinaris (LcH, Lentil) (FITC)
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Technique
FITC, Fluorescein
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Host
Rabbit
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Isotype
IgG
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Label
FITC
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Alternative name
Lens culinaris (LcH, Lentil) (fluorecein)
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Alternative technique
fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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