Human Recombinant PKLR
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Catalog number6373-50
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PricePlease ask
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Size50 ug
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SynonymsHuman Recombinant PKLR
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Alternative_namesPyruvate kinase isozyme R/L, PK1, PKL, PKR, PKRL, RPK.
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DescriptionCatalyzes the rate-limiting step of glycolysis
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Biological activitySpecific activity: >0.1 unit/mg.
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Results>0.1 unit/mg
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Unit DefinitionOne unit will form 1.0 µmol of phosphoenolpyruvate to pyruvate per minute at pH 7.5 at 37°C.
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Molecular Weight59.2 kDa (549 aa, 47-574 aa + NT His-Tag)
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form description1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 1 mM DTT and 10% glycerol.
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Background InformationPKLR is a pyruvate kinase which catalyzes the trans phosphorylation of Phosphoenolpyruvate into pyruvate and ATP. That is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. The L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.
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Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolPKLR
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Short nameRecombinant PKLR
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameH. sapiens Rec. PKLR
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namepyruvate kinase L/R
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Synonyms gene name
- pyruvate kinase, liver and RBC
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data