D-Serine Dehydratase, Active, Recombinant
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Catalog numberP1193-200
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PricePlease ask
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Size200 ug
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SynonymsDsdSC, D-serine deaminase, DSD, DSD1
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Alternative_namesDsdSC, D-serine deaminase, DSD, DSD1
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DescriptionConverts D-serine to pyruvate and ammonia
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RecombinantYes
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SourceE.coli
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Purity by SDS PAGE> 95%
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AssaySDS-PAGE
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Activity Specifications test methodMeasured by its ability to metabolizes 1 umole of D-serine into pyruvate per minute at 37°C and pH 7.4, in the presence of pyridoxal-5-phosphate and catalytic Zn2+.
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Biological activitySpecific activity is ≥ 60 mU/mg
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Results≥ 60 mU/mg
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Unit DefinitionOne Unit of DsdSC metabolizes 1 umole of D-serine into pyruvate per minute at 37°C and pH 7.4, in the presence of pyridoxal-5-phosphate and catalytic Zn2+.
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Molecular Weight47.8 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 50 mM Sodium phosphate, pH 7.0, 30% glycerol
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Reconstitution InstructionsReconstitute in 50 mM potassium phosphate, 50 mM NaCl, pH 7.4.
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Background InformationD-Serine Dehydratase belongs to the β- family of the Pyridoxal phosphate-dependent enzymes and part of the fold-type II family. It binds to one catalytically essential PLP per protein molecule but also contains another low affinity PLP binding site. The enzyme converts D-Serine, D-threonine, D-allo-Threonine to corresponding α-keto acid and ammonia. It is suggested to act as a detoxifying enzyme for E.Coli strains.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Additional descriptionSerine protease, D- or L-serine arginine rich enzyme of serine threonine kinase with serine that is encoded by the codons UCU, UCC, UCA, UCG, AGU and AGC is an ɑ-amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated −NH+ 3 form under biological conditions), a carboxyl group. It is non-essential in humans, meaning the body can synthesize it.
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Gene target
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Short nameD-Serine Dehydratase, Active, Recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative nameD-Serine Dehydratase, functionnal, Rec.
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Alternative techniquerec
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MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data