Human CellExp™ KIM3/HAVCR2, human recombinant
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Catalog number7495-10
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Price:
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Size10 ug
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SynonymsHAVCR2, TIM3, TIMD3, FLJ14428, KIM3
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Alternative_namesHAVCR2, TIM3, TIMD3, FLJ14428, KIM3
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DescriptionA Th1-specific cell surface protein that regulates macrophage activation.
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥97%
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AssaySDS-PAGE
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Biological activityMeasured by its binding ability in a functional ELISA. Immobilized rh-Galectin-9 at 500 ng/ml can bind rhKIM3-Fc with an apparent KD
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ResultsMeasured by its binding ability in a functional ELISA. Immobilized rh-Galectin-9 at 500 ng/ml can bind rhKIM3-Fc with an apparent KD
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Molecular WeightThis protein is fused with the Fc region of human IgG1 at the C-terminus and has a calculated MW of 42 kDa after removal of signal peptide. In DTT-reduced SDS-PAGE, rhKIM3-Fc protein migrates as 45 kDa poly peptide due to glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationHepatitis A virus cellular receptor 2 also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolHAVCR2
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Short nameCellExp™ KIM3/HAVCR2, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ KIM3/hepatitis A virus cellular receptor 2, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targethepatitis A virus cellular receptor 2, HAVCR2 and IDBG-55271 and ENSG00000135077 and 84868, protein binding, Plasma membranes, Havcr2 and IDBG-165782 and ENSMUSG00000020399 and 171285
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Gene info
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Identity
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Gene
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Long gene namehepatitis A virus cellular receptor 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-12-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data