Human CellExp™ IL-12, Mouse Recombinant

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• Catalog number
  4719-100
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• Size
  100 ug

# #

• Synonyms
  NKSF, CLMF
• Alternative_names
  NKSF, CLMF, Cytotoxic lymphocyte maturation factor 40 kDa subunit
• Description
  A T cell-stimulating factor
• Recombinant
  Yes
• Source
  HEK 293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Purity
  ≥95%
• Biological activity
  The activity is determined by the induced expression of CD25 on CD8+ mouse T cells and is typically detectable starting at 0.1 ng/mL.
• Results
  0.1 ng/mL
• Molecular Weight
  70 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Recombinant mouse IL-12 is lyophilized from 0.5 x PBS, pH 7.5.
• Reconstitution Instructions
  Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
• Background Information
  Interleukin-12 (IL-12) is an important mediator of cellular-immunity produced by dendritic cells, macrophages and B cells when stimulated with antigen. IL-12 is involved in differentiating precursor helper T cells to Th1 and/or Th2 subtypes and promotes the production of IFN gamma and TNF alpha. Mouse IL-12 is active on human and mouse cells. Recombinant mouse IL-12 is a glycosylated, disulfide-linked heterodimer. It is containing one p35 subunit (35 kDa) and one p40 subunit (40 kDa), with a total of 506 amino acids and a molecular weight of approximately 70 kDa.
• Amino acid sequence
  p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

#

• Gene target
  CellExp™   IL-12  
• Gene symbol
  IFNLR1, PIRC57, PIRC55, PIRC54, PIRC53, PIRC56, SNORD115-12, SNORD114-12, IGHV3OR16-12
• Short name
  CellExp™ IL-12, Mouse Recombinant
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  mouse
• Species
  Humans, Mouses
• Alternative name
  H. sapiens CellExp™ Interleukin-12, Mouse Rec.
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:18584
• Gene
  IFNLR1
• Long gene name
  interferon lambda receptor 1
• Synonyms gene
  • IL28RA
• Synonyms gene name
  • interleukin 28 receptor, alpha
  • interleukin 28 receptor, alpha (interferon, lambda receptor)
  • interferon, lambda receptor 1
• Synonyms
  • CRF2/12
  • IFNLR
  • IL-28R1
• GenBank acession
  • AY129153
• Locus
  1p36.11
• Discovery year
  2002-12-02
• Entrez gene record
  163702
• RefSeq identity
  • NM_170743
• Classification
  • Interferon receptors
• VEGA ID
  OTTHUMG00000003036

Gene info

• Identity
  HGNC:37561
• Gene
  PIRC57
• Long gene name
  piwi-interacting RNA cluster 57
• Locus
  12
• Discovery year
  2009-11-05
• Entrez gene record
  100313930
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37559
• Gene
  PIRC55
• Long gene name
  piwi-interacting RNA cluster 55
• Locus
  12
• Discovery year
  2009-11-05
• Entrez gene record
  100313792
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37558
• Gene
  PIRC54
• Long gene name
  piwi-interacting RNA cluster 54
• Locus
  12
• Discovery year
  2009-11-05
• Entrez gene record
  100313815
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37557
• Gene
  PIRC53
• Long gene name
  piwi-interacting RNA cluster 53
• Locus
  12
• Discovery year
  2009-11-05
• Entrez gene record
  100313861
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37560
• Gene
  PIRC56
• Long gene name
  piwi-interacting RNA cluster 56
• Locus
  12
• Discovery year
  2009-11-05
• Entrez gene record
  100313862
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:33031
• Gene
  SNORD115-12
• Long gene name
  small nucleolar RNA, C/D box 115-12
• Synonyms
  • HBII-52-12
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033449
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003304.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33000
• Gene
  SNORD114-12
• Long gene name
  small nucleolar RNA, C/D box 114-12
• Synonyms
  • 14q(II-12)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767590
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003205.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5636
• Gene
  IGHV3OR16-12
• Long gene name
  immunoglobulin heavy variable 3/OR16-12 (non-functional)
• Synonyms gene name
  • immunoglobulin heavy variable 3/OR16-12
• Synonyms
  • IGHV3/OR16-12
• GenBank acession
  • Z29609
• Locus
  16p11.2
• Discovery year
  2000-04-17
• Entrez gene record
  28304
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000176355

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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