Human CellExp™ EpCAM/CD326, human recombinant
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Catalog number7450-10
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Price:
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Size10 ug
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SynonymsEPCAM, DIAR5, DIAR-5, EGP-2, EGP2, EGP314, EGP-314, EGP40, EGP-40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1/4, KSA, M4S1, MIC18, MIC-18, MK-1, MK1, TACSTD1, TACSTD-1, TROP1, TROP-1, CD326, CD-326, Epithelial cell adhesion molecule, tumor-associated calcium signal transducer 1, cluster of differentiation 326.
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Alternative_namesEPCAM, DIAR5, DIAR-5, EGP-2, EGP2, EGP314, EGP-314, EGP40, EGP-40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1/4, KSA, M4S1, MIC18, MIC-18, MK-1, MK1, TACSTD1, TACSTD-1, TROP1, TROP-1, CD326, CD-326, Epithelial cell adhesion molecule, tumor-associated calcium signal transducer 1, cluster of differentiation 326.
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DescriptionA pan-epithelial differentiation antigen that is expressed on almost all carcinomas
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activityMeasured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 ug/mL (100 ul/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.
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ResultsMeasured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 ug/mL (100 ul/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.
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Molecular WeightThis protein contains a poly histidine tag at C-terminus and has a calculated MW of 29 kDa after removal of signal peptide. The predicted N-terminus is Gln24. In DTT-reduced SDS-PAGE, rh EpCAM protein migrates as 33-36 kDa poly peptide due to glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationEpCAM is also known as CO171A,; EGP; EGP40; GA7332; KSA; M4S; MIC18; MK1; TROP1; hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolEPCAM
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Short nameCellExp™ EpCAM/CD326, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ epithelial cellular adhesion molecule/CD326, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetepithelial cell adhesion molecule, DIAR5 and EGP-2 and EGP314 and EGP40 and ESA and HNPCC8 and KS1/4 and KSA and M4S1 and MIC18 and MK-1 and TACSTD1 and TROP1, EPCAM and IDBG-50831 and ENSG00000119888 and 4072, protein complex binding, Cell surfaces, Epcam and IDBG-201437 and ENSMUSG00000045394 and 17075, EPCAM and IDBG-633617 and ENSBTAG00000006474 and 514039
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Gene info
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Identity
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Gene
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Long gene nameepithelial cell adhesion molecule
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Synonyms gene
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Synonyms gene name
- antigen identified by monoclonal antibody AUA1
- tumor-associated calcium signal transducer 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-10-02
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Entrez gene record
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Pubmed identfication
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Classification
- MicroRNA protein coding host genes
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data