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Synonyms
NRP1, Neuropilin-1, NRP, VEGF165R, CD304
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Alternative_names
NRP1, Neuropilin-1, NRP, VEGF165R, CD304
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Description
A receptor involved in the development of the cardiovascular system
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Recombinant
True
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Source
HEK 293 cells
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Purity by SDS PAGE
>90%
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Assay
SDS-PAGE
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Endotoxin Level
Less than 0.1 EU per ug by the LAL method.
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Activity Specifications test method
Immobilized Human VEGF165, Tag Free at 2 ug/mL (100 uL/well) can bind Mouse NRP1, Mouse IgG2a Fc Tag with a linear range of 0.5-4 ng/mL
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Binding Capacity
Immobilized Human VEGF165, Tag Free at 2 ug/mL (100 uL/well) can bind Mouse NRP1, Mouse IgG2a Fc Tag with a linear range of 0.5-4 ng/mL
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Molecular Weight
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 120.5 kDa. The protein migrates as 125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Neuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.
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Amino acid sequence
AA Phe 22 - Pro 856
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Handling
Centrifuge the vial prior to opening.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NRP1
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Short name
CellExp™ CD304 / NRP1 / Neurophilin-1, Fc Tag, mouse recombinant
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Technique
Recombinant, Mouse, FC, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
Fc Tag
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Species
Humans, Mouses
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Alternative name
H. sapiens CellExp™ CD304 / neuropilin 1 / Neurophilin-1, fragment c detection labelled, mouse Rec.
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Alternative technique
rec, murine
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Alternative to gene target
neuropilin 1, BDCA4 and CD304 and NP1 and NRP and VEGF165R, NRP1 and IDBG-68532 and ENSG00000099250 and 8829, metal ion binding, Extracellular, Nrp1 and IDBG-201088 and ENSMUSG00000025810 and 18186, NRP1 and IDBG-637309 and ENSBTAG00000019866 and 539369
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