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Synonyms
Angiopoietin-related protein 7, ANGPTL7, CDT6, Angiopoietin-like factor, Angiopoietin-like protein 7, Cornea-derived transcript 6 protein
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Alternative_names
Angiopoietin-related protein 7, ANGPTL7, CDT6, Angiopoietin-like factor, Angiopoietin-like protein 7, Cornea-derived transcript 6 protein
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Description
Growth factor that enhance the expansion and engraftment of hematopoietic stem cells
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
> 85%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Human ANGPTL7, His Tag at 5 ug/mL (100 uL/well) can bind Human LILRB4, Fc Tag with a linear range of 0.031-0.5 ug/mL Immobilized Human ANGPTL7, His Tag at 5 ug/mL (100 uL/well) can bind Human LILRB2, Fc Tag with a linear range of 0.031-2 ug/mL Immobilized Human ANGPTL7, His Tag at 5 ug/mL (100 uL/well) can bind Human LILRA3, Fc Tag with a linear range of 0.016-1 ug/mL
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Binding Capacity
Immobilized Human ANGPTL7, His Tag at 5 ug/mL (100 uL/well) can bind Human LILRB4, Fc Tag with a linear range of 0.031-0.5 ug/mL Immobilized Human ANGPTL7, His Tag at 5 ug/mL (100 uL/well) can bind Human LILRB2, Fc Tag with a linear range of 0.031-2 ug/mL Immobilized Human ANGPTL7, His Tag at 5 ug/mL (100 uL/well) can bind Human LILRA3, Fc Tag with a linear range of 0.016-1 ug/mL
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Molecular Weight
39 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to a concentration up to 0.2 mg/ml.
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Background Information
Angiopoietin-like 7 (ANGPTL7) is also known as Corneal-Derived Transcript 6 (CDT6), and is a secreted glycoprotein that is structurally related to the angiopoietins. It is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
