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Verified reactivity
Horse
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Detection limits
31.25 pg/ml-2000 pg/ml
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Sensitivity limit
7,81 pg/ml
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Samples to be analyzed
serum, plasma, tissue homogenates
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Assay duration
1-5 hours
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Verified applications
ELISA
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Assay class
Please contact us
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ELISA detection
Colorimetric
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Protein number
Q16665
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Alternate protein number
Please refer to SwissProt
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Gene number
Please refer to GenBank
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Gene name
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A)
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Alternate gene name
Please refer to NCBI
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Estimated production time
7-11 business days
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Shipping requirements
Blue ice
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Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for
6 months
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ELISA s specificity
To know the specificity of this test please feel free to contact us
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ELISA s cross reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principle
Please see ELISA's datasheet, otherwise contact us
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Protocol
Please see ELISA's datasheet, otherwise contact us
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Precision of the test
Please see ELISA's datasheet, otherwise contact us
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ELISA s stability
Please see ELISA's datasheet, otherwise contact us
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QC
The kit is manufactured at ISO 9001 certified facilities.
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Research main area
Epigenetics and Nuclear Signaling
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Warnings
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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Notes
For research use only. Not for diagnostic procedures.
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Description
The hypoxia factor 1, alpha subunit ( helix-loop-helix transcription factor) (HIF1A) ELISA Kit, 96 is a α- or alpha protein sometimes glycoprotein present in blood. Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
HIF1A-AS3, HIF1A-AS1, HIF1A-AS2, HIF1A
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Short name
hypoxia factor 1, alpha subunit ( helix-loop-helix transcription factor) (HIF1A) ELISA Kit, 96
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
Horse, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Horse, Horses
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Alternative name
Horse hypoxia inducible factor 1, a functionnal sequence (basic helix-loop-helix transcription factor) (hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)) Enzyme-linked immunosorbent assay reagent, 96 well plate
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Alternative technique
elisas, kits
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Alternative to gene target
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), bHLHe78 and HIF-1A and HIF-1alpha and HIF1 and HIF1-ALPHA and MOP1 and PASD8, HIF1A and IDBG-8510 and ENSG00000100644 and 3091, Hsp90 protein binding, nuclei, Hif1a and IDBG-148620 and ENSMUSG00000021109 and 15251, HIF1A and IDBG-646757 and ENSBTAG00000020935 and 281814
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