-
Products_type
Antibody
-
Products_short_name
[CD74]
-
Products_name_syn
[CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain]
-
Other_names
[HLA class II histocompatibility antigen gamma chain; CD74 molecule]
-
Products_gene_name
[CD74]
-
Other_gene_names
[CD74; II; DHLAG; HLADG; Ia-GAMMA]
-
Clonality
Monoclonal
-
Clone
[CLIP/1133]
-
Reactivity
Human
-
Specificity
Recognizes proteins of 33, 35 and 41kDa, which are identified as various isoforms of CD74. Its epitope is localized in the extracellular domain of CD74. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, and dendritic cells and by various types of carcinomas. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
-
Form
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
-
Storage_stability
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
-
Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Description
For cells, cell lines and tissues in culture till half confluency.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
CD74
-
Short name
CD74 (B- ) Mouse Monoclonal Antibody
-
Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA. Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
-
Host
Mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
Isotype
IgG1, kappa
-
Species
Mouse, Mouses
-
Alternative name
CD74 molecule, major histocompatibility complex, class II invariant chain (B-cellular detection labelled) Mouse monoclonal (antibody to-)
-
Alternative technique
antibodies, murine
-
Alternative to gene target
CD74 molecule, major histocompatibility complex, class II invariant chain, DHLAG and HLADG and Ia-GAMMA and II, CD74 and IDBG-53594 and ENSG00000019582 and 972, MHC class II protein binding, Cell surfaces, Cd74 and IDBG-150302 and ENSMUSG00000024610 and 16149, BT.46809 and IDBG-646288 and ENSBTAG00000015228 and 613384
-
Tissue
cell
-
