HEPES, cell culture tested - 250g
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Catalog numberPM-B2093/250
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Price:
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Size3X250g
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DescriptionHEPES, cell culture tested - 250g is a white free-flowing crystalline powder with the molecular formula C8H18N2O4S and that is used in cell culture media.
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Shipping conditions-20°C or dry ice
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StorageRoom Temperature
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Shelflife36 months
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StateLyophilized
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Chemical descriptionMiscellaneous products
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AppearancePhysical state at 20 ºC: Solid ; Appearance: Crystalline ; Colour: White with coloured particles
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Density at 20 ºC1440 kg/m³
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Relative density at 20 ºC1.44
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PHNA
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OsmolalityNA
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EndotoxinNA
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CompositionNA
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SterilityNon sterile
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Personal precautionsWear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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UsesUsed in biotechnology. For professional user/industrial user only.
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OrderingTo order HEPES, cell culture tested - 250g, please use the Cat. Nr.PM-B2093/250 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for HEPES, cell culture tested - 250g
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Additional descriptionFor cells, cell lines and tissues in culture till half confluency.
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Gene target
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Short name, culture tested - 250g
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Techniqueculture
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Alternative nameHEPES, cellular culture tested - 250g
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Tissuecell
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MeSH Data
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Name
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ConceptScope note: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
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Tree numbers
- E05.481.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data