H4K20me3 polyclonal antibody
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Catalog number
6877-25
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Price
Please ask
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Size
25 μg
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Antibody Target
H4K20me3
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Target Alternative Name
Histone 4
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Antibody Type
Polyclonal
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Species Reactivities
Human.
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Immunogen Sequence
Polyclonal antibody raised in rabbit against the region of histone H4 containing the trimethylated lysine 20 (H4K20me3), using a KLH-conjugated synthetic peptide.
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Accession
P68431
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Gene Id
8350
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
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Purification
Affinity Purified
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Application
Western blot, IF, ELISA, Dot Blot, ChIP.
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Positive Control
Western blot: HeLa cells, IF: U2OS cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides.
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Application And Usages
WB: 1:1000, Dot Blot: 1:20,000, ChIP: 1-2 µl/ChIP, IF: 1:300, ELISA: 1:100.
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Description
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Verified
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ChIP Verified
Verified
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Dot Blot Verified
Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Short name
H4K20me3 polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Rabbit IgG
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Alternative name
H4K20me3 polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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