Guinea Pig Polyclonal IgG Anti Elastin human

Guinea Pig Polyclonal IgG Anti Elastin human is available 2 times from Bio logo labs

EL25013 | Guinea Pig Polyclonal IgG Anti-Elastin, human size: 0.5 ml | 733.08 USD

  • Catalog number
    EL25013
  • Supplier
  • Price
    733.08 USD
  • Size
    0.5 ml
1. Gene info
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
Additional information
Product images

EL25013-0.1 | Guinea Pig Polyclonal IgG Anti-Elastin, human size: 0.1 ml | 370.59 USD

Ajax processing
  • Protein concentration
    app. 1 mg/ml
  • Antibody's isotype
    IgG
  • Positive control
    Human skin or aorta
  • Specificity
    Human Elastin 100%, human Collagen Type I, II, III, IV, V
  • Product is supplied as
    purified, lyophilized
  • Immunogen
    Purified elastin from human skin
  • Species reactivity
    Human, cross-rectivity with rat Elastin < 0.1%
  • Pretreatment
    After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  • Tested applications
    IFA, IHC(P), ELISA, RIA
  • Availability
    Please contact us to check the availability
  • Storage method
    Store at -20°C
  • Antibody's raised in
    Guinea Pig
  • Antibody's clone
    polyclonal
  • Litterature
    1. Demarchez M., Hartmann D.J., Herbage D., Ville G., and Prunieras M. (1987) Wound-healing of human skin transplanted onto the nude mouse. 2. an immunohistochemical and ultrastructural study of the epidermal basment-membrane zone reconstruction and connenc 2. Demarchez M., Hartmann D.J., and Prunieras M. (1987) An immunohistochemical study of the revascularization process in human skin transplanted onto the nude mouse. Transplantation 43; 896-903. 3. Lethias C., Hartmann D.J., Masmejean M., Ravazzola M., Sabbagh I., Ville G., Herbage D., Eloy R. (1987) Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique. J. Histochem. Cytochem. 35, 15-21.
  • Product category
    Primary Antibody
  • Product presentation
    Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
  • Recommended concentration for use
    IFA ≥ 1:80, IHC(P) ≥ 1:500 - 1:1000, ELISA ≥ 1:200 (OD ≥ 500)
  • UniProt number
    P155Go to UniProt/SwissProt2 (ELN_HUMAN)
  • Recommended secondary reagent
    Anti-Guinea pig IgG-conjugates
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Pigs and the smaller guinea pigs are frequent used as models for humans.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • Additional isotype
    IgG
  • Group
    Polyclonals and antibodies
  • Gene target
  • Short name
    Polyclonal IgG Anti-Elastin,
  • Technique
    Polyclonal, anti, IgG
  • Isotype
    IgG
  • Species
    Human
  • Alternative name
    Guinea swine polyclonal Immunoglobulin G antibody to-Elastin, H. sapiens
  • Alternative technique
    polyclonals
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