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Available ordering format
Lyophilized
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Immunogen
Purified Rabbit IgG (H&L)
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified goat IgG, F(ab)'2 fragment
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How to reconstitute
For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
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Connected products
other antibodies to rabbit IgG HRP conjugated
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Recommended dilutions for use
optimal working dilution should be determined by the investigator
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
rabbit IgG (H&L)
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Possible reactivity
rabbit IgG (H&L)
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
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References
Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.
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Scientific context
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
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Notes
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated