Glucose Uptake Fluorometric Assay Kit
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Catalog numberK666-100
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Price:
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Size100 assays
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DescriptionNon-radioactive, Sensitive Assay
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Summary• Detection method- Fluorescence (Ex/Em = 535/587 nm) • Species reactivity- Mammalian • Applications- This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
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Sample TypeCell lysates
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Features Benefits• Simple procedure • Fast and convenient • The assay is easy, non-radioactive, and highly sensitive
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundGlucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to BioVision’s PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Short nameGlucose Uptake Fluorometric Assay Kit
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TechniqueAssay, assays
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HostAssay
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Alternative nameGlucose Uptake fluorescence test reagent
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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