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Alternative Name
Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light; polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10; NFKB2; LYT10
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Immunogen
Synthetic phosphopeptide derived from human NFkB-p100 around the phosphorylation site of Serine 865.
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Swiss Prot
Q00653
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Reactivity
Human,Mouse,Rat
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Applications
WB IHC
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Application_all
WB: 1:500~1:1000 IHC: 1:50~1:200
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
p-NFkB-p100 (S865) polyclonal antibody pAb detects endogenous levels of NFkB-p100 protein only when phosphorylated at Ser865.
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Usage
Western blot (WB) analysis of NFkB-p100 (phospho-S865) polyclonal antibody at 1:500 dilution Lane1:NIH-3T3 whole cell lysate(40ug) Lane2:NIH-3T3(PMA, 60ng/ml, 5min)whole cell lysate(40ug) Lane3:NIH-3T3(PMA, 60ng/ml, 15min)whole cell lysate(40ug) Lane4:NIH-3T3(PMA, 60ng/ml, 30min)whole cell lysate(40ug) Lane5:NIH-3T3(EGF, 0.1ng/ml, 30min)whole cell lysate(40ug)
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BiowMW
~ 100, 120 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
