GAPDH, Active, human recombinant
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Catalog numberP1100-10
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PricePlease ask
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Size10 ug
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SynonymsGlyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD
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Alternative_namesGlyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD
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DescriptionInvolved in glycolysis
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Activity Specifications test methodMeasured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C
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Biological activitySpecific activity is > 90 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
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Results> 90 units/mg
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Unit DefinitionOne unit is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
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Molecular Weight36 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form descriptionIn 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20% glycerol
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Background InformationGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques.
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Amino acid sequenceMGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolGAPDH-DT, GAPDH, GAPDHS, GAPDHP15
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Short nameGAPDH, Active, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameGAPDH, functionnal, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameGAPDH divergent transcript
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Locus
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Discovery year2021-03-12
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Entrez gene record
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Classification
- Divergent transcripts
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase
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Synonyms gene
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase, spermatogenic
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase pseudogene 15
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Synonyms gene
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Synonyms
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Locus
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Discovery year2003-11-26
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Entrez gene record
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data