Isocitrate Dehydrogenase 1 R132H mutant, Recombinant Human
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Catalog numberP1070-10
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Price:
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Size10 ug
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SynonymsIsocitrate dehydrogenase [NADP] cytoplasmic, R132H, IDH 1 R132H
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Alternative_namesIsocitrate dehydrogenase [NADP] cytoplasmic, R132H, IDH 1 R132H
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DescriptionCatalyzes the rate-limiting step in Citric acid cycle
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Biological activity≥ 300 mU/mg, in which one unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25 ̊C.
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Results≥ 300 mU/mg
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Unit DefinitionOne unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25 ̊C.
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Molecular Weight50.9 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Reconstitution InstructionsReconstitute in deionized water to the final concentration 0.5 mg/ml and incubate the reconstituted protein at 25 °C for 15 minutes.
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Background InformationMutations of the cytosolic IDH 1 are a common feature in primary human brain cancers. Arginine 132 (R132) of IDH is highly conserved among different isoforms of IDH and is most commonly mutated to Histidine. Mutation of IDH1 R132H leads to accumulation of R(-)-2-hydroxyglutarate (2HG), which correlates with an increased risk for malignant brain tumors.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Short nameIsocitrate Dehydrogenase 1 R132H mutant, Recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameIsocitrate Dehydrogenase 1 R132H mutant, Rec. H. sapiens
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Alternative techniquerec
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MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data