FractionPREP™ Cell Fractionation Kit

Summary

• Species reactivity- Mammalian • Applications- 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Sample Type

Cell cultures (adherent and suspension), fresh and frozen tissues
Features Benefits

• Simple procedure; takes less than 1 hour • Fast and convenient • The kit is designed for serial sample preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions, from one sample.
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

This FractionPREP™ Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Data Sheet Link
Additional description

For cells, cell lines and tissues in culture till half confluency.

Gene target

FractionPREP™ Fractionation Kit
Short name

FractionPREP™ Fractionation Kit
Alternative name

FractionPREP™ cellular Fractionation reagent
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Tissue

cell