FITC-PEG-Biotin,1K
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Catalog numberFL044041-1K
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Price:
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Size100mg
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Product categoryFluorescent PEG derivatives
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Availability and orderingPlease contact Gentaur for the latest availability and to request a quote. For larger or bulk quantity a discount may apply
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Technical datasheetPlease contact our technical support every day between 9 am to 6 pm local time to request the latest datasheet, certificate of analysis and MSDS
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This Biochempeg Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biochempeg FITC is highly sensitive to pH extremes.
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ConjugationBiotinylated, Anti-FITC Antibody
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Gene target
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Short nameFITC-PEG- ,1K
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TechniqueFITC, Fluorescein
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LabelFITC
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Alternative namefluorecein-PEG-biotinilated,1K
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Alternative techniquefluorescine
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data