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Alternative_name
FITC-Leu-Glu-His-Asp-fluoromethyl ketone; FITC-L-E(OMe)-HD(OMe)-FMK
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Description
FITC-LE(OMe)HD(OMe)-FMK is a fluorescein isothiocyanate (FITC) conjugate of the cell- permeable caspase-9 inhibitor LE(OMe)HD(OMe)-FMK that acts as an in situ marker for detection of caspase-9 in living cells. FITC-LE(OMe)HD(OMe)-FMK is nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells. The bound marker is localized by fluorescence detection.
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Structure Available
Yes
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Peptide sequence
FITC-L-E(OMe)-HD(OMe)-FMK
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Salt Form
No
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Molecular Formula
C₄₅H₄₈FN₇O₁₃S
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Molecular Weight
945.97
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Cell Permeable
Yes
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Purity
≥98% by HPLC
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Handling
Protect from air and moisture
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Tag Line
A caspase-9 in situ marker
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
36 months
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SMILES
S/C(N[C@@H](CC(C)C)C(N[C@H](C(N[C@@H](CC1=CN=CN1)C(N[C@H](C(CF)=O)CC(OC)=O)=O)=O)CCC(OC)=O)=O)=N\C2=CC=C(C3(C4=CC=C(O)C=C4OC5=C3C=CC(O)=C5)OC6=O)C6=C2
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InChi
InChI=1S/C45H48FN7O13S/c1-22(2)13-33(41(60)50-31(11-12-38(57)63-3)40(59)52-34(15-24-20-47-21-48-24)42(61)51-32(35(56)19-46)18-39(58)64-4)53-44(67)49-23-5-8-28-27(14-23)43(62)66-45(28)29-9-6-25(54)16-36(29)65-37-17-26(55)7-10-30(37)45/h5-10,14,16-17,20-22,31-34,54-55H,11-13,15,18-19H2,1-4H3,(H,47,48)(H,50,60)(H,51,61)(H,52,59)(H2,49,53,67)/t31-,32-,33-,34-/m0/s1
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InChi Key
QDHULOWFRSEJMR-CUPIEXAXSA-N
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MSDS Available
Yes
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Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
