-
Long name
FAK(Tyr861) Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
-
Also known as
Anti-FAKTyr861 PAb ALEXA FLUOR 350
-
Category
Conjugated Primary Antibodies
-
Conjugated with
ALEXA FLUOR® 350
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
FAKTyr861
-
Specificity
This is a highly specific antibody against FAKTyr861.
-
Modification
Phosphorylation
-
Modification Site
Tyr861
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Immunogen range
840-890/1052
-
Subcellular location
Cytoplasm, Nucleus, Cell membrane
-
Source
KLH conjugated synthetic phosphopeptide derived from human FAK around the phosphorylation site of Tyr861
-
Gene ID Number
5747
-
Swiss Prot
Q05397
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human, Mouse, Rat
-
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the antigen
Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL.
-
Purification
Purified by Protein A.
-
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
-
Excitation emission
343nm/442nm
-
Synonyms
FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2
-
Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Conjugation
Alexa Fluor, ALEXA FLUOR 350
-
French translation
anticorps
