Methanol Assay Kit (Colorimetric)
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Catalog number
K898-100
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Price
Please ask
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Size
100 assays
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Description
Market's first and only kit for the enzymatic detection of Methanol in biological samples
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Summary
• Detection method-Absorbance (450 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other samples • Species reactivity- Mammalian /others • Application- The kit detects 500 ppm Methanol
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Detection Method
Absorbance (450 nm)
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Species Reactivity
Mammalian /others
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Applications
Determination of methanol in biological samples, anaerobic bacterial cultures, agricultural products and food products
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Sample Type
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological samples
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Features Benefits
• Simple procedure; takes ~40 minutes - 1 hour • Fast and convenient • Kit contains the necessary reagents for accurate and specific measurement of Methanol in biological samples
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Methanol is the simplest alcohol, consisting exclusively of a methyl group and a hydroxyl moiety. It is both an important industrial molecule (solvent, fuel, building block for chemical synthesis) and a biological metabolite; many bacteria generate methanol as a result of anaerobic metabolism. In humans, ingestion of large quantities of methanol is toxic and suppresses the nervous system; this is termed ‘methanol poisoning’. As such, methanol is frequently used as an additive in industrial alcohols to prevent human consumption. The toxicity of methanol is primarily due to the biological product of its metabolism, formaldehyde, which is further metabolized into formic acid. At lower concentrations, methanol poisoning can cause loss of coordination and discomfort, and at higher concentrations will lead to kidney failure, blindness, and even death. In the gut, microbial breakdown of pectin-rich foods produces small amounts of methanol. In addition, some bacteria are capable of metabolizing methane gas, generating methanol, and so monitoring methanol concentration is also relevant for industrial purposes and renewable energy research. BioVision’s Methanol Assay Kit utilizes an enzymatic mechanism by which conversion of methanol is correlated stoichiometrically with generation of a colorimetric signal that can be quantified at 450 nm. The assay shows greater than 100-fold specificity for methanol over ethanol. The method is suitable for use in a range of biological and consumable samples, and can detect as little as 500 pmol methanol.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Methanol Assay Kit (Colorimetric)
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Methanol test reagent (colorimetry)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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