EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric)
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Catalog number
P-4032-96
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Price
Please ask
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Size
96 Assays
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Note
For research use only.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Description
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
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Test
Chromatin isolation for histone extracts like H2A, H2B, H3, and H4 extraction for SDS-PAGE electrophoresis. Histones act as spools around which DNA winds, and also play a role in gene regulation. The core histones include H2A, H2B, H3, and H4. Histones undergo post-translational modifications, which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have long tails protruding from the nucleosome, which can be covalently modified at several places (i.e., methylation, acetylation, phosphorylation, etc.). Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis). Combinations of modifications are thought to constitute a "histone code" and an understanding of this code may help to answer questions about human development and disease.
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Gene target
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Short name
EpiQuik Histone H4 Acetylation Fast Kit (Colorimetric)
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Technique
colorimetric, detection, Epiquik, colorimetry, epiquik
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Alternative name
EpiQuik Total Histone H4 Acetylation quantification Fast reagent (colorimetry)
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Alternative technique
assays, epigenetic, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
total
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