Epigen (EPGN), human recombinant
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Catalog number
4969-100
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Price
Please ask
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Size
100 ug
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Synonyms
EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
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Alternative_names
EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
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Description
Promotes the growth of epithelial cells
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Purity
≥98%
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Biological activity
Determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expected ED₅₀ for this effect is 150-300 ng/ml.
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Results
150-300 ng/ml
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Molecular Weight
7.9 kDa
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Storage Temp
-20°C
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Shipping
Blue ice
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Sterile filtered and lyophilized from 20 mM PBS buffer pH-7.4 containing 130 mM sodium chloride
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Reconstitution Instructions
Reconstitute in sterile dH₂O not less than 100 ug/ml.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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Background Information
EPGN is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. EPGN is produced in numerous tissues, including the testis, liver, heart and in certain tumor cells. EPGN is mitogenic for fibroblasts and epithelial cells. Human EPGN is originally synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant Human epigen produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9 kDa. Epigen is purified by proprietary chromatographic techniques.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
EPGN
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Short name
Epigen (EPGN), recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Epigen (EPGN), H. sapiens Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
epithelial mitogen
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Synonyms gene name
- epithelial mitogen homolog (mouse)
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Synonyms
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Synonyms name
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Locus
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Discovery year
2006-10-17
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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